This page contains various protocols I’ve learned during my journey as a scientist. Pages will be updated over time.
- Chlorophyll-A extraction
- Media preparation
- Check plating
- Stress tests
- Growth Curve
- Phenol:Chloroform DNA/RNA extraction
- Primer design
- PCR
- Gel electrophoresis
- DNA clean-up and concentration
- Preparation of chemically competent E. coli
- Cloning I (digestion)
- Cloning II (ligation)
- Cloning III a. (transformation)
- Cloning III b. (electroporation)
- Colony PCR
- Microscopy
- Protein-protein interactions (bacterial two-hybrid)
- Protein induction
- Protein purification
- Protein gel preparation
- Western blot
- Lipid extraction (Bligh & Dyer)
- Lipid droplet purification
- Lipid droplet protein purification
- Thin-layer chromatography
- Gas chromatography-mass spectrometry
- Bioinformatics